Primary structure of phage mu transposase: homology to mu repressor.
نویسندگان
چکیده
منابع مشابه
Carboxyl Terminal Truncations of the Phage Mu Transposase
The Phage Mu transposase (MuA) is a 663 amino acid momomeric multidomain protein. A tetramer of MuA catalyzes the replicative transposition of Phage Mu. MuA pairs the ends of the Mu genome, cleaves the donor DNA and subsequently joins the Mu DNA to the target DNA in a concerted cleavage and joining reaction known as strand transfer. A series of truncations deleting 101, 136 and 173 amino acids ...
متن کاملSolution structure of the I gamma subdomain of the Mu end DNA-binding domain of phage Mu transposase.
The MuA transposase of phase Mu is a large modular protein that plays a central role in transposition. We show that the Mu end DNA-binding domain, I beta gamma, which is responsible for binding the DNA attachment sites at each end of the Mu genome, comprises two subdomains, I beta and I gamma, that are structurally autonomous and do not interact with each other in the absence of DNA. The soluti...
متن کاملSolution structure of the Mu end DNA-binding ibeta subdomain of phage Mu transposase: modular DNA recognition by two tethered domains.
The phage Mu transposase (MuA) binds to the ends of the Mu genome during the assembly of higher order nucleoprotein complexes. We investigate the structure and function of the MuA end-binding domain (Ibetagamma). The three-dimensional solution structure of the Ibeta subdomain (residues 77-174) has been determined using multidimensional NMR spectroscopy. It comprises five alpha-helices, includin...
متن کاملMutational analysis of the att DNA-binding domain of phage Mu transposase.
The transposase (A protein) of phage Mu encodes binding to two families of DNA sites, att sites located at the Mu ends and enhancer sites located internally. Separate subdomains in the N-terminal domain I of Mu A protein are known to be involved in recognition of the att and enhancer sites. We have delineated an approximately 135 aa region within domain I beta gamma that specifies binding to Mu...
متن کاملStructural domains in phage Mu transposase: identification of the site-specific DNA-binding domain.
Limited proteolysis of phage Mu transposase with three proteases of differing specificities produced a common pattern of fragmentation. The fragments were mapped by using a combination of immunoblotting and amino acid sequence analysis. Our results suggest that the transposase molecule is organized principally into three domains: an amino-terminal domain of molecular mass 30 kDa, a core region ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1985
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.82.22.7676